MLMC Frontiers: Multi-Dimensional Microscopy

Montreal Light Microscopy Course – Advanced Level Course Overview

The August 2016 MLMC is designed to provide one week of advanced instruction in optical microscopy. Students, fellows, PIs, technical staff, core facility managers, and research scientists are welcome to attend. Lectures will be presented in the morning, while the afternoons will be devoted towards hands-on sessions with a variety of optical microscopy techniques.

Lectures will be presented by experts in optical microscopy drawn from the academic, government, and industrial sectors. Afternoon laboratory sessions build on the morning lectures. Coffee breaks and lunch, as well as social activities and the Keynote Lecture, are included.

MLMC Location

Charles F. Martin Amphitheatre
McIntyre Medical Sciences Building
3655 Promenade Sir William Osler, Montreal H3G 1Y6



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History of the MLMC

The First MLMC – 2010

The first Montreal Light Microscopy Course (MLMC) was held July 5-16, 2010 at McGill University. The course was organized by Dr. Claire M. Brown, director of the McGill University Imaging Facility, Dr. Pina Colarusso, scientific director of the Live Cell Imaging Facility at the University of Calgary, and Dr. Paul Maddox, of the Department of Pathology and Cell Biology and the Institute for Research in Immunology and Cancer (IRIC) at Université de Montréal.

The course was billed a great success with about 80 students participating. Participants came from across Canada (Ottawa, Montreal, Saskatoon, Calgary, Halifax, Newfoundland), from the US (Vermont) and from Europe (Sweden). Over half of the participants had the privilege of gaining extensive hands-on access to state-of-the-art microscopy equipment during the course.

During the introductory first week of the course (Fundamentals) students were exposed to the microscopy theory that is the foundation for all modern microscopes. Participants were given many opportunities to put those theories into practice using some of more than 30 different systems that were available during the course. Many of these systems were transported to Montreal and installed onsite specifically for the MLMC. During the more advanced second week of the course (Frontiers) participants were able to gain hands-on access to state-of-the-art equipment, some of which is not even readily available on the market. This provided a peak into what technologies are at the frontier of light microscopy. Some highlights included imaging live specimens at speeds that were preciously not possible without the rapid developments in confocal, camera and filter technologies. New cameras and super-resolution techniques were also a highlight, producing images with resolutions approaching that of electron microscopes by using novel light microscopy techniques that can break the diffraction barrier.

Over thirty companies and approximately sixty representatives travelled from across North America and Europe in order to participate in the course. The highlight was a remote presentation from the Netherlands on deconvolution via Skype and TeamViewer software. The pinnacle of the course was the keynote presentation by Dr. Sergio Grinstein from the Toronto Sick Kids Hospital. He so eloquently brought together a number of his research stories about cellular phagocytosis and intertwined the stories with results from the many light microscopy techniques he uses in his research. This truly demonstrated to the course attendees the power of light microscopy to effectively address complex research questions.

Feedback ...

  • “It was a learning experience that I will never forget.” Joseph Clarke (PhD Student, Memorial University)
  • “Thank you so much for this great program! I now know what can potentially be achieved with microscopy based approaches.” Emmanuel Levy (Post Doctoral Fellow, Université de Montréal)
  • “I must say that I have done a lot of courses in my days and this one was exceptional. You all did a beautiful job.” Michael Stanley, PhD, Senior Applications Scientist, Chroma Technologies Inc.
  • “The small groups and small number of people allowed for individual tailoring.” Anonymous
  • “Great course! I learned a lot and I am very happy that I had attended it.” Anonymous

For information, please contact: mlmc@curlydog.ca
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